November 20, 2013

 

Research provides recommendations to improve monitoring for the detection of avian mycoplasmas

 

 

A research by the University of Georgia provides recommendations to improve the monitoring system to detect mycoplasmas, allowing the poultry industry to maximise the benefits of costly diagnostic assays and set scientifically based standards in different laboratories for sample submission and handling.

 

Control of the most important avian mycoplasmas, Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) is of great importance to the poultry industry and is addressed in the research.

 

In the research project funded by the USPOULTRY Foundation, Dr Naola Ferguson-Noel from the University of Georgia determined the effects of transport media type and sample swab type on the sensitivity of the real-time polymerase chain reaction (PCR) for MS and MG. Her recommendations will help improve the sensitivity of the test and provide standardisation to sample collection and transport.

 

Currently, the approach to Mycoplasma gallisepticum (MG) and M. synoviae (MS) control in the United States includes continuous surveillance of breeding flocks to ensure early detection of infection. It is of utmost importance that an early test for mycoplasmosis is as sensitive as possible, as missed infections can lead to more opportunities for horizontal and vertical spread of these economically devastating diseases.

 

Although advances in real-time polymerase chain reaction (PCR) technology have resulted in extremely sensitive and fast tests that are affordable and amenable to high throughput testing, the added sensitivity of real-time PCR may be severely reduced due to inappropriate handling and processing of swabs.

 

The main goal of this project was to identify optimum methods for collection and handling of tracheal swabs for real-time PCR detection of MG and MS. The specific objectives of this study are: 1) to evaluate two commercially available liquid media for potential MG and MS real-time PCR inhibition; 2) to compare the sensitivity of pre-moistened and dry tracheal swabs for MG and MS real-time PCR; and 3) to evaluate the effect of storage temperature and time on dry and moist swabs for MG and MS real-time PCR.

 

Research findings show that neither of the commercially available media tested (Liquid Amies or Liquid Stuarts) inhibited the real-time PCR for MG or MS to any great degree.

 

The Remel BactiSwab® swab transport system and its equivalent dry swab were tested and the results indicated that pre-moistened swabs may collect more mycoplasma cells and thereby enhance the sensitivity of real-time PCR.

 

The quantity of DNA detected from tracheal swabs (dry and wet) declined over time regardless of storage 40 C or room temperature. The decline was greatest in wet samples stored at room temperature and least in dry swabs stored at 40 C. Dry tracheal swabs are the best option for routine sampling for real-time PCR, based on convenience and stability, especially when swabs may take some time to reach the laboratory.

 

Although pre-moistened tracheal swabs do not store as well as dry swabs, they may be useful in difficult cases (e.g. chronic infections, antibiotic treatment) where maximum sensitivity is required. It is even more important that pre-moistened swabs are handled properly. The tracheal swabs should be kept cool and processed as soon as possible for optimum results.

 

The research project is part of USPOULTRY and the USPOULTRY Foundation's research programme on all phases of poultry and egg production and processing and is funded by these bodies.