August 6, 2018


Injectable trace minerals reduce oxidative stress after aflatoxin challenge in dairy cows


In a new study, researchers at the University of Illinois investigated the potential of injectable trace minerals in dairy cows to reduce the damage from consuming aflatoxin-contaminated feed.


"If we're providing enough trace minerals to manufacture more detoxifying enzymes, the liver has a better chance of fighting the aflatoxin," explains Russell Pate, doctoral student in the Department of Animal Sciences at U of I and lead author on the study, to


Phil Cardoso, assistant professor in the department and co-author on the paper, adds that an injectable product could be helpful during aflatoxin challenge, when a cow's appetite is suppressed. "Our idea for injecting is that it might help during a time when intake is impacted. If something is causing her to eat less, she'll consume less of any diet-based mineral too."


The researchers injected lactating Holsteins with a commercially available injectable trace mineral formulation, twice, about a month apart. Another set of animals received a saline injection only, but both sets received the recommended amount of trace minerals in their diet. About a month after the second injection, the researchers introduced aflatoxin-contaminated feed to half of the animals.


Cows receiving aflatoxin and saline had greater liver inflammation and lower feed efficiencies than cows that were not exposed to aflatoxin. For the set of aflatoxin-challenged cows which received the injectable trace mineral formulation, liver concentrations of selenium and iron increased. The injectable also raised the activity of serum glutathione peroxidase, which protects cells from oxidative damage by neutralizing free-radicals.


"As we had hypothesized, supplying cows with trace minerals via injection, independent of minerals ingested in the feed, resulted in an improved immune response and reduced oxidative stress when cows were challenged with aflatoxin," Pate says. Trace minerals are co-factors of enzymes responsible for alleviating the oxidative stress in animals.


However, Aflatoxin M1 was present in all milk samples for cows that received the contaminated feed. In other words, injectable trace minerals did not prevent transfer of the toxin into the milk. The researchers were not surprised by the result, though.


"We knew it would not help with transference to the milk. If the cow is exposed, she needs to excrete that toxin through milk, urine, faeces; anywhere to get rid of it," Cardoso explains. "To stop transference to the milk, you have to first identify the source of the aflatoxin and avoid feeding it. But sometimes you just can't. In those cases, we are showing for the first time that an injectable trace mineral will provide a benefit."


The article, "Injectable trace minerals (selenium, copper, zinc, and manganese) alleviates inflammation and oxidative stress during an aflatoxin challenge in lactating multiparous Holstein cows," is published in the Journal of Dairy Science.